sensitive detection of giardia cysts by polymerase chain reaction (pcr)
نویسندگان
چکیده
giardia is one of the most common human parasites and causes a lengthly course of nonbacterial diarrhea. disease outbreaks due to giardia infection are often attributed to contaminated water supplies. a major problem associated with detection for this organism is the lack of sensitive and reliable methods. pcr has the potential to address many of the limitations.we have performed a pcr-based method for sensitive detection of giardia cysts. because the sensitivity of pcr is a function of the efficiency of dna extraction from cysts, we have also compared some different methods for dna extraction from the cysts. giardia cysts were collected from infected human, partially purified and serially diluted samples were prepared. dna was extracted by 3 different methods and we found that simple repeated freezing and thawing was the best method for extraction of dna from the cysts. a 163 bp conserved fragment related to the giardial heat shock protein (hsp70) gene was used as the target for pcr amplification. we were able to detect as few as 5 cysts in the samples. the results suggest the potential utilities of pcr for sensitive detection of giardia in water sources.
منابع مشابه
Detection of giardine gene in local isolates of Giardia duodenalis by polymerase chain reaction (PCR).
Giardia duodenalis is an intestinal parasite that causes diarrhoea and malabsorption in children. The parasite also infects AIDS patients with a weak immune system. A study was carried out on six local isolates of Giardia duodenalis (110, 7304, 6304, M007, 2002 and 6307) from faeces of Orang Asli patients admitted to the Gombak Hospital. WB, a reference pathogenic strain from human and G. muris...
متن کاملPolymerase Chain Reaction (PCR)
The polymerase chain reaction (PCR) is a powerful and widely used technique that has greatly advanced our ability to analyze genes. Genomic deoxyribonucleic acid (DNA) present in cells contains many thousands of genes. This makes it difficult to isolate and analyze any individual gene. PCR allows specific DNA sequences, usually corresponding to genes or parts of genes, to be copied from genomic...
متن کاملDetection of Chlamydia trachomatis Using polymerase chain reaction (PCR)
Chlamydia trachomatis now is one of the most prevalent bacteria sexually transmissible diseases (STD), and as such, constitutes a serious public health problem. Diagnosis of Chlamydial infections is based on isolation of bacteria in tissue culture media that requires at least 48 to 72h. The aim of this study was the detection of Chlamydia trachomatis, using polymerase chain reaction (PCR), beca...
متن کاملA Rapid and Sensitive Detection of Aflatoxin-producing Fungus Using an Optimized Polymerase Chain Reaction (PCR)
Aflatoxin B1 (AFB1) is produced by Aspergillus flavus growing in feedstuffs. Early detection of maize contamination by aflatoxigenic fungi is advantageous since aflatoxins exert adverse health effects. In this study, we report the development of an optimized conventional PCR for AFB1 detection and a rapid, sensitive and simple screening Real-time PCR (qPCR) with SYBR Green and two pairs of prim...
متن کاملRapid Detection of Campylobacter jejuni by Polymerase Chain Reaction and Evaluation of its Sensitivity and Specificity
Introduction: Campylobacter jejuni is one of the most common causes of food poising in humans. Rapid and specific detection of these bacteria has an important role in diagnosis and treatment of infection. The aim of this study was to design a specific PCR for the detection of Campylobacter jejuni. Methods: In this experimental study, oxidoreductase gene from the Campylobacter jejuni was sele...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian journal of public healthجلد ۳۲، شماره ۱، صفحات ۱۵-۱۸
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023